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The Human Immune Monitoring Center (HIMC) is designed to be a comprehensive source for immune monitoring services for clinical and translational studies. We perform: Immunoassays: Luminex cytokine arrays, MesoScale Discovery assays Mass Cytometry (CyTOF): Immunophenotyping, Intracellular cytokines, Phospho-epitopes Flow Cytometry: Immunophenotyping, Intracellular cytokines, Phospho-epitopes Genomics: RNA/DNA extractions, Microarrays, Microfluidic qPCR arrays, Targeted RNAseq BioBanking: Serum, Plasma, PBMC, and Tissue processing; Sample banking and retrieval
Parallel coordinates view of Luminex cytokine data: SPADE analysis of CyTOF data: ![]() ![]() Goals of the HIMC include:
The HIMC was developed in 2007 by the Institute for Immunity, Transplantation, and Infection (Mark Davis, Ph.D., director) in conjunction with the Center for Clinical Immunology (C. Garrison Fathman, founder). Our Faculty Advisor is Mark Davis.
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Holden Maecker, PhD | Facility Director
Office (Rm D039, Fairchild Building): (650) 723-1671
Email: maecker at stanford.edu
Name |
Role |
Phone |
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Location |
Yael Rosenberg-Hasson |
Technical Director, Immunoassay Mgr |
650-723-4984 |
yaelhr at stanford.edu |
Fairchild Science Bldg, D033 |
Mike Leipold |
Mass Cytometry (CyTOF) Manager |
650-723-4984 |
mleipold at stanford.edu |
Fairchild Science Bldg, D033 |
Rosemary Fernandez |
Flow Cytometry Manager |
650-723-4984 |
rfernan at stanford.edu |
Fairchild Science Bldg, D033 |
Xuhuai Ji |
Genomics Manager |
650-723-5050 |
xuhuai.ji at stanford.edu |
CCSR, 0128 |
Rohit Gupta |
Biobank Director |
650-724-9062 |
rogupta at stanford.edu |
3165 Porter Drive, Rm 2122 |
Stephanie Neudecker |
Lab Manager | 650-723-4984 | sneudeck at stanford.edu | Fairchild Science Bldg, D033 |
Location |
Building |
Room |
Hours |
Location |
Immunoassay & Cell Analysis Lab |
Fairchild Science Bldg |
D033 |
9 AM - 5 PM |
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Genomics Lab |
CCSR |
0128 |
8 AM - 5 PM |
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BioBanking Lab |
3165 Porter Dr. |
2185 |
9 AM - 5 PM |
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As with all Stanford Service Centers, credit must be given to Human Immune Monitoring Center (HIMC) for data that results in a publication. If the work done at HIMC produces data resulting in a figure in a publication, you are required to acknowledge HIMC in the publication. Further, if HIMC staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.
One of our CyTOFs was purchased with funds from a NIH S10 Shared Instrumentation Grant (S10RR027431-01). Please acknowledge this grant in your papers using CyTOF data.
Name | Role | Phone | Location | |
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Holden Maecker, PhD |
Director
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650.723.1671
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maecker@stanford.edu
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Fairchild Sci Bldg, Rm D039
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Debra Panasuk |
Finance Manager
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650.725.7483
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dpanasuk@stanford.edu
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1215 Welch Road, Modular A, Rm 96
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FY16 Service Price List |
► Biobanking (11) | |||
Name | Description | Price | |
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Biobank Setup & Maintenance | Inquire | ||
Custom Materials - Biobanking | Inquire | ||
PBMC processing (CPT tubes) | Inquire | ||
PBMC processing (HEP) | Inquire | ||
PBMC processing from tissue | Inquire | ||
Plasma processing | Inquire | ||
Sample banking (per vial) | Inquire | ||
Serum processing | Inquire | ||
Technical Consulting or Labor Time (BioBanking Courier) - per hour | Inquire | ||
Technical Consulting or Labor Time (Biobanking General) - per hour | Inquire | ||
Technical Consulting or Labor Time (Biobanking Sample Retrieval) - per hour | Inquire | ||
► Flow Cytometry (9) | |||
Name | Description | Price | |
CyTOF ICS (per stim) | (view additional details) | Inquire | |
CyTOF instrument use (trained users) - per hour | Inquire | ||
CyTOF instrument use+tech labor (assisted run) - per hour | Inquire | ||
CyTOF phenotyping | (view additional details) | Inquire | |
CyTOF phospho-flow (per stim) | (view additional details) | Inquire | |
Flow phenotyping (Lyoplate) | (view additional details) | Inquire | |
ICS (LSRII) (per stim) | (view additional details) | Inquire | |
Phospho-flow | (view additional details) | Inquire | |
Technical Consulting or Labor Time (Flow Cytometry) - per hour | Inquire | ||
► Immuno Assays (8) | |||
Name | Description | Price | |
Luminex human 62-plex | Inquire | ||
Luminex mouse 38-plex | Inquire | ||
HAI titer | Inquire | ||
MSD Meso Scale Discovery (Multiplex Elisa) assay - Materials | (view additional details) | Inquire | |
MSD Meso Scale Discovery (Multiplex Elisa) assay - Tech Labor, per hour | (view additional details) | Inquire | |
Custom Materials - Immuno Assays | (view additional details) | Inquire | |
Technical Consulting or Labor Time (Immuno Assays) - per hour | Inquire | ||
Elispot Independent Use - per hour | Inquire | ||
► Molecular and Genomic Assays (14) | |||
Name | Description | Price | |
2nd color labeling for microarray | Inquire | ||
Bioanalyzer DNA/RNA | Inquire | ||
Custom Materials - Genomics | Inquire | ||
Fluidigm BioMark qPCR array (48.48) | Inquire | ||
Gene Array Data Analysis Service (Tech Labor) - per hour | Inquire | ||
HIMChip | Inquire | ||
miRNA array (Agilent) | Inquire | ||
NuGen cDNA amplification | Inquire | ||
PAXgene DNA extraction | Inquire | ||
PAXgene RNA extraction | Inquire | ||
Targeted RNAseq (per 96-well plate) | Inquire | ||
Technical Consulting or Labor Time (Molecular and Genomic Assays) - per hour | Inquire | ||
Trizol RNA or DNA | Inquire | ||
Whole genome GE array (Agilent) | Inquire | ||
► Other Services and Fees (4) | |||
Name | Description | Price | |
Custom Materials Charge | Inquire | ||
Shipping from Stanford FedEx Charge | Inquire | ||
Shipping to Stanford FedEx Charge | Inquire | ||
Technical Consulting or Labor Time (Elispot Training) - per hour | Inquire |