Welcome!
Welcome to Transgenic, Knockout, and Tumor Model Center
Partly supported by funds from Stanford Cancer Institute, the Stanford Transgenic, Knockout, and Tumor Model Center is set to produce your transgenic, knockout and cancer mouse models. The facility provides low-cost DNA microinjection services, ES cell manipulation, microinjection and tumor modeling services to all Stanford as well as external investigators. The facility also provides services on timed pregnant females, strain rederivation, embryo and sperm cryopreservation and IVF procedures for resurrecting frozen sperm. The Center can also provide transgenic animal tumor models and a variety of dosing/surgical services for in vitro and in vivo tumor studies.
Dr. Vittorio Sebastiano is the Director of the facility with responsibility for its daily operation. Dr. Barry Behr is the Faculty Advisor who oversees the strategic direction of the facility. The facility has an advisory board consisting of Dr. Julien Sage (Pediatrics), Dr. Sherri Green (Comparative Medicine), and Dr. Dean Felsher (Oncology). Oversight is also provided by the Cancer Institute senior leadership to ensure that the needs of Cancer Institute members are met. Dr. Hong Zeng is senior Research Scientist, Dr. Jeewon Kim is a Research Scientist; Jennifer Lin, Judy Song ,Yanfeng Li, and Wei Chen are research staff of the facility.
All Stanford investigators who have approved A-PLAC (Administrative Panel on Laboratory Animal Care) protocol numbers are eligible to use the facility's services. The facility has an approved protocol #12002 which covers the production of transgenic and knockout mice and protocol #19267 which covers the production of tumor-bearing mice. Investigators only need protocols for characterization of the transgenic and chimeric animals. It usually takes 4-6 weeks to get a protocol approved by A-PLAC; so you should plan ahead and have a protocol number ready when requesting the service.
External users who have an approved animal protocol and animal housing location are also eligible to use the services provided by the TKTC.
If you have questions regarding animal protocols, you may contact Dr.Claude Nagamine at 498-4773 or Valerie Fratus at 723-4550.
Users not paying with an internal Stanford PTA will have to pay a Facilities and Administration (F&A) charge of 58% (starting 1 Sept. 2015). Exceptions will be made for payments from Stanford Affiliates (organizations with a formal affiliation agreement with Stanford) and for payments from U.S. accredited, degree-granting institutions of higher education using their own institution’s non-sponsored funding (who will pay 8% F&A).
Vittorio Sebastiano, Ph.D. | TKTC Director
Office: (650)
Email: vsebast at stanford.edu
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Location |
Hours |
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Transgenic, Knockout, and Tumor Model Center Stanford University Medical Center Room 0137, CCSR Building Stanford, CA 94305 Tel: (650) 725-6871 |
9 a.m. - 5 p.m., Monday to Friday
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Visit our facility website for more information: http://med.stanford.edu/transgenic/
As with all Stanford Service Centers, credit must be given to Stanford Transgenic, Knockout, and Tumor Model Center (TKTC) for data that results in a publication. If the work done at Stanford TKTC produces data resulting in a figure in a publication, you are required to acknowledge Stanford TKTC in the publication. Further, if Stanford TKTC staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Vittorio Sebastiano, Ph.D. |
Director
|
vsebast at stanford.edu
|
SIM1 G3078A
|
|
| Hong Zeng, Ph.D. |
Sr. Scientist and manager
|
650-724-9556
|
hongzeng at stanford.edu
|
CCSR#0137A
|
| Jeewon Kim, Ph.D. |
Research Scientist
|
650-721-1485
|
jwonkim at stanford.edu
|
CCSR 0137
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| Primary Contact - TKTC |
(650) 725-6871
|
CCSR Room 0137
|
