Stanford University Mass Spectrometry

 Welcome to the Vincent Coates Foundation Mass Spectrometry Laboratory.

The laboratory is named in honor of a generous gift from Vincent and Stella Coates, given through the Stanford School of Medicine to support the mass spec & proteomics facility as a shared core resource. It is a Bio-X core facility, embodying the Bio-X spirit of interdisciplinary communication and collaboration. It is also partially supported as the Proteomics Shared Resource of the Stanford Cancer Institute. The Dean of Research provides oversight and support to keep the laboratory's expertise and support available to researchers throughout Stanford University & Medical Center.

Save the date! 

2016 SUMS-RAS
Thursday October 6th
Frances C. Arrillaga Alumni Center

Overview of Services

Beyond making available state-of-the-art, user-friendly facilities and services, the laboratory enables education, methods development, and new applications development, designed to meet the rapidly evolving needs of researchers. Due to the essential information that mass spectrometry provides to researchers in the fields of the physical and life sciences, medicine, and engineering, the laboratory serves as an “intellectual watering hole” at the crossroads of diverse disciplines.

At this time, we have in operation three GC-MS and ten LC-MS systems -- four single quads, one ion trap, four triple quads, one hybrid quadrupole-time of flight, one benchtop Orbitrap and two hybrid LTQ-Orbitraps -- as well as a MALDI-TOF. Software resources include instrument-specific packages Xcalibur, MassLynx, ChemStation; proteomics software Byonic, Mascot, Bioworks, Sequest Sorcerer, Scaffold; and other tools Mass Frontier, QuanLynx, OpenLynx, MetaboLynx, ChemDraw.

Routine mass spec and proteomic services include molecular weight determination, MSn, LC-MS, high resolution MS, bottom-up proteomics, and more. Please contact SUMS to discuss custom work such as complex proteomic analysis, relative and targeted quantitation, drug discovery support, metabolomics, de novo peptide sequencing, new application development, and other projects.

Types of Service and Analysis Offered by SUMS:

• Proteomics
• Protein identification - gel or solution based
• Shotgun proteomics, e.g. cell lysates, tissue preps
• Analysis of frozen or FFPE banked tissues
• Post-translational modification studies, e.g. phosphorylation, methylation, ubiquitination
• Quantitative studies - labeled and unlabeled differential expression; targeted absolute quantitation
• Protein characterization - intact mass, peptide mapping

• Small Molecules
• Molecular weight confirmation
• High resolution molecular formula confirmation (HRMS)
• Structural elucidation (MSn)
• Quantitation

• Drug Discovery Support
• Pharmacokinetic (PK) assessment
• Microsome incubations
• Metabolite identification
• Stability assessment
• Formulation assessment
• Degradation studies

• Assays & Quantitation
• For small molecules and proteins/peptides
• Pre-established assays for select classes of molecules
• Custom assay development for molecules of interest

• Metabolomics
• Targeted, Quantitative
• Semi-Quantitative

• Open access (Stanford Users Only)
• LC-MS
• GC-MS
• MALDI-TOF

• Other custom projects - contact SUMS to discuss

Getting Started

• Stanford Users:  LOGIN or REGISTER for an iLab account using valid Stanford credentials (SUNet ID).

• Non-Stanford Users:  Click to LOGIN with approved iLab credentials. Or, click to SIGN UP for an iLab account.

• Visit the Customer Help Manual for further guidance using iLab's Core Facility Services.

Leadership

Allis Chien, PhD | SUMS Director

Office (Mudd 167A):  (650) 723-0710

Lab    (Mudd 175):  (650) 725-9769

Email:  allis at stanford.edu

Staff

Lab Email:  spectrometry@stanford.edu

Location and Hours of Operation 

Click to view SUMS location on Stanford Campus Map

Links and Resources

• Visit our lab website for more information: https://mass-spec.stanford.edu/

Publication Acknowledgement 

In accordance with Dean's office policy: If work done with SUMS produces data in a publication, you must acknowledge us ("Vincent Coates Foundation Mass Spectrometry Laboratory, Stanford University Mass Spectrometry") in the publication. Further, if SUMS staff provide significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.  

In addition, the LTQ Orbitrap Velos was obtained via an NIH instrumentation grant, and the NIH requires that each publication, press release or other document that uses data from the LTQ-Orbitrap Velos instrument must include an acknowledgement of NIH grant support and disclaimer as follows:
"The project described was supported by Award Number S10RR027425 from the National Center For Research Resources. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center For Research Resources or the National Institutes of Health."

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