Functional Genomics Facility

Overview of Services

The Stanford Functional Genomics Facility (SFGF) provides assays, services, and equipment for cutting edge genetic and genomic based research. 

Available Services: (New services)

• High-throughput Sequencing and Library Construction utilizing Illumina MiSeq, NextSeq, HiSeq 2000, and HiSeq 4000
• Single-Cell RNA-Seq utilizing 10x Genomics Chromium 
• Gene Expresion Assays - Microarray Platforms: Affymetrix, Illumina, Agilent
• Whole Genome Genotyping Assays - Microarray Platforms: Illumina and Affymetrix
• Karyotyping (Illumina)
• Gene Expression and Genotyping using real-time PCR 
• Digital PCR (BioRad)
• Nanostring single molecule counting
• Bioanalyzer DNA, RNA and Protein analysis, Qubit quantification, DNA shearing on Covaris
• Sample Tracking, Sample Contamination and Cell Line Authentication Services
• TALEN construction
• Custom Array Printing of any material
• Preferred pricing on Custom Oligonucleotides from IDT - Stanford-IDT Portal
• Streamlined ordering and preferred pricing for Oligo Pools from Agilent

Available Equipment 24/7:  (You can request 24/7 access to the following equipment by going to the Request Services tab and selecting SFGF 24/7 Access)

• Real-time PCR:  ABI 7900HT, ABI StepOnePlus, Roche LightCycler 480, Stratagene MX 3005p
• Microarray Scanners:  Agilent Scanner, Axon Scanner
• Imagers:  GE Typhoon TRIO, LiCOR Odyssey, Alpha Imager
• Quanitation:  Bioanalyzer, Nanodrop, Qubit 2.0 and 3.0 Fluorometers
• Automation:  Biomek FX, Microarray Printer, MAUI 12 Bay Hybridization System, NuGEN Mondrian
• General Equipment:  Tetrad PCR Machines 

Getting Started

• Stanford Users:  LOGIN or REGISTER for an iLab account using valid Stanford credentials (SUNet ID).

• Non-Stanford Users:  Click to LOGIN with approved iLab credentials. Or, click to SIGN UP for an iLab account.

• Visit the Customer Help Manual for further guidance using iLab's Core Facility Services.

Leadership

John Coller, PhD | SFGF Director

Office: CCSR 0120  -  (650) 736-1026

Lab: CCSR 0120  -  (650) 736-2434

Email:  john.coller at stanford.edu

Staff

Location and Hours of Operation

Links and Resources

• Visit our facility website for more information: http://www.microarray.org/

Publication Acknowledgement 

As with all Stanford Service Centers, credit must be given to the Stanford Functional Genomics Facility for data that results in a publication. If the work done at SFGF produces data resulting in a figure in a publication, you are required to acknowledge the Stanford Functional Genomics Facility in the publication. Further, if SFGF staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication. 

The Illumina HiSeq 4000 was purchased using a NIH S10 Shared Instrumentation Grant (S10OD018220).  Please acknowledge this grant in any publications using data from this instrument.

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Contacts